Future feed control – Tracing banned bovine material in insect meal

In the present study, we assessed if different legacy and novel molecular analyses approaches can detect trace prohibited bovine material in insects reared to produce processed animal protein (PAP). Newly hatched black soldier fly (BSF) larvae were fed one of four diets for seven days; a control feeding medium (Ctl), feed spiked with hemoglobin powder (BvHb) at 1% (wet weight, w/w) (BvHb 1%, w/w), 5% 5%, 10% 10%, w/w). Another dietary group BSF larvae, namely *BvHb was first grown on BvHb (w/w), after days separated from residual placed another container diet additional days. Presence ruminant insect five laboratories using (i) real time-PCR analysis, (ii) multi-target ultra-high performance liquid chromatography coupled tandem mass spectrometry (UHPLC-MS/MS), (iii) protein-centric immunoaffinity-LC-MS/MS, (iv) peptide-centric (v) spectral library matching (SLM), (vi) compound specific amino acid analysis (CSIA). All methods investigated detected DNA or media respectively. However, each method assessed, displayed distinct shortcomings, which precluded detection versus non-prohibited some instances. Taken together, these findings indicate that insect-PAP chain requires tiered combined use complementary approaches. We therefore advocate multi-tier suite detection, differentiation tracing based chains endorse ongoing efforts extend currently available battery PAP MS techniques possibly ?13CAA fingerprinting.